Biochemical /Biophysical Methods
Please acknowledge the SIP core facility ( RRID: SCR_018986) in publications, on posters, or in talks if you use any instruments in the SIP core facility. Please include SIP's RRID (RRID: SCR_018986) and the grant numbers for instruments funded through instrumentation grants in your acknowledgements. This is a requirement from the funding agencies and is crucial for future funding. Find example text on theÌýÌýor the individual instrument pages.
SIP offers cutting-edge instruments, techniques and tools, along with expert support, to ensure thorough biophysical and biochemical analysis of biomolecules, complexes, and biochemical reactions. Our state-of-the-art techniques include:
Spectroscopy
- X-Band, CW Electron Paramagnetic Resonance Spectroscopy
- Fluorescence Spectroscopy (Intensity, Fluorescence Polarization / Anisotropy, Fluorescence Energy Transfer)
- Circular Dichroism Spectroscopy
- UV/Vis spectroscopy
Fast Kinetics
- Stopped-flow Spectroscopy in Fluorescence (Intensity, FP/Anisotropy and FRET-based), Circular Dichroism and UV/VIS mode
- Chemical Quench-flow Analysis
Sizing and Oligomerization
- Dynamic Light Scattering
- Size Exclusion Chromatography Multi-Angle Light Scattering
- Continuous Gradient Multi-Angle Light Scattering
- Mass Photometry
Binding studies
- Isothermal Titration Calorimetry
- Microscale Thermophoresis
- Fluorescence Polarisation / Anisotropy, FRET or Intensity (cuvette or plate reader based)
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