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Biomolecular X-ray Crystallography

The Biomolecular X-Ray Crystallography Lab provides resources for crystallization and structure determination of biological macromolecules.  Three controlled-temperature rooms (20 °C, 4 °C and 30 °C) are available for crystallization experiments.  Two data collection systems, with cryo-cooling, are available for crystal characterization, cryoprotectant screening, and in-house data collection. One data collection system consists of a Rigaku MM007 rotating anode x-ray generator with Varimax optics, a Raxis IV++ image plate detector, and an Oxford cryosystem. The second system is a XtaLAB MM003 equipt with a MicroMax-003 Microfocus sealed tube X-ray generator system, a AFC11 Partial-χ, 4-axis goniometer, a Dectris PILATUS 200K 2D hybrid pixel array area detector and an Oxford Cryosystems Cryostream Unit. Resources are available for remote data collection at synchrotrons, as well as for in-house crystallographic computation and model-building. In addition, the lab houses a Phoenix dropsetter for crystallization and two RockImager system for crystal imaging.

Contact: Annette Erbse - 303-492-0528

Central Analytical Mass Spectrometry Lab

We specialize in proteomics-based mass spectrometry analyses that implement high-resolution liquid chromatography separations and instruments capable of high resolution, accurate mass determinations.  We routinely provide bioinformatics data processing for all datasets.  We also provide additional analyses including, but not limited to, the identification and quantitation of small organic molecules, oligonucleotides, peptides, proteins and polymers using a variety of instruments and mass spectrometry-based methods.

The High Throughput Screening Core Facility is equipped with instrumentation for high-throughput and high-content biochemical and cell-based assays, including live-cell assays. Lab Director Xiang Wang is available to support the drug discovery process, including data analysis and lead identification and validation. The facility also offers a series of viability assays and human cancer cell lines.

The NMR Facilities at the University of Colorado Boulder maintains a variety of state-of-the-art NMR spectrometers at a variety of magnetic fields to support the wide variety of research needs of the entire University. The NMR spectrometers available for hands-on use are located in Room 02 in the Cristol Chemistry Building.